Practical work 1: Detection of electroporation on microorganisms by fluorescent dyes (epifluorescent microscope, fluorometer, flow citometer).

Practical work 2: Detection of electroporation in plant cells (impedance measures, conductivity, release of intracellular liquid by centrifugation).

Practical work 3: Influence of different parameters on microbial inactivation by PEF (Electric field strength, number of pulses, temperature, pH, type of microorganisms).

Practical work 4: Influence of processing parameters on PEF-assisted extraction (Electric field strength, number of pulses).

Practical work 5. Pilot plant demonstrations.